alexa fluor 488  (Bioss)

Journal: Materials Today Bio

Article Title: Targeted biomimetic fusogenic liposome enhances tumor accumulation, penetration, and therapeutic efficacy of siRNA therapeutics

doi: 10.1016/j.mtbio.2025.102414

Figure Lengend Snippet: Antitumor effects of CRPIL/siCD47/siPLK1 complexes. (a). Expression of CD4 7 mRNA and PLK1 mRNA in 4T1 cells after different treatments. (b) The phagocytosis efficiency of J774A.1 cells against 4T1 cells after different treatments. (c) Schematic illustration of treatment schedule. (d) Changes in tumor volumes during the treatment. (e–f) Photographs of dissected tumors and average tumor weights at the end of the treatment. (g) Expression of CD47 and PLK1 proteins in tumor tissues after different treatments. (h) Representative images of hematoxylin and eosin-stained, TUNEL-stained and calreticulin (CRT)-stained tumor tissues on day 14.

Article Snippet: After washing, the membranes were incubated with CD47 Polyclonal antibody (Proteintech Group, 20305-1-AP), PLK1 Polyclonal antibody (Proteintech Group, 10305-1-AP) or GAPDH Monoclonal antibody (Proteintech Group, 60004-1-Ig) for 1 h at room temperature.

Techniques: Expressing, Staining, TUNEL Assay

Journal: The EMBO Journal

Article Title: CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2

doi: 10.1038/s44318-024-00240-z

Figure Lengend Snippet: Reagents and tools table

Article Snippet: PLK1-pT210 polyclonal (Rabbit, 1:500) , CST , Cat #5472S, RRID:AB_10698594.

Techniques: Recombinant, Plasmid Preparation, Sequencing, CRISPR, Control, Protease Inhibitor, Transfection, Software, Protein Purification, Mutagenesis, Mass Spectrometry, Western Blot